abstract：:Human endothelin-1 (ET-1) is a potent vasocontractile 21-residue peptide hormone with significant pharmacological importance. An efficient and straightforward expression strategy that enables cost-effective incorporation of stable isotopes is not available thus far. In this report, we describe a cost-effective express...

journal_title：Protein expression and purification

authors： Mac TT,Beyermann M,Pires JR,Schmieder P,Oschkinat H

更新日期：2006-08-01 00:00:00

abstract：:An improved, simplified method for the purification of recombinant, tagged DnaA proteins is described. The presented protocol allowed us to purify soluble DnaA proteins from two different bacterial species: Helicobacter pylori and Streptomyces coelicolor, but it can most likely also be used for the isolation of DnaA p...

journal_title：Protein expression and purification

authors： Zawilak-Pawlik AM,Kois A,Zakrzewska-Czerwinska J

更新日期：2006-07-01 00:00:00

abstract：:Silent information regulator 2 (Sir2) proteins are a class of protein deacetylase enzymes that play key roles in transcriptional gene silencing, DNA repair, and aging. Here, we describe the high-level bacterial expression and purification of a human SirT2 construct that yields high resolution NMR spectra. By removing ...

journal_title：Protein expression and purification

authors： Sun C,Song D,Marcotte PA,Richardson PL,Hajduk PJ

更新日期：2006-07-01 00:00:00

abstract：:Conkunitzin-S1 from the cone snail Conus striatus is the first member of a new neurotoxin family with a canonical Kunitz domain fold. Conk-S1 is 60 amino acids long and lacks one of the three conserved disulfide bonds typically found in Kunitz domain modules. It binds specifically to voltage activated potassium channe...

journal_title：Protein expression and purification

authors： Bayrhuber M,Graf R,Ferber M,Zweckstetter M,Imperial J,Garrett JE,Olivera BM,Terlau H,Becker S

更新日期：2006-06-01 00:00:00

abstract：:Antimicrobial peptide LL-37 plays an important role in human body's first line of defense against infection. To better understand the mechanism of action, it is critical to elucidate the three-dimensional structure of LL-37 in complex with bacterial membranes. We present a bacterial expression system that allows the i...

journal_title：Protein expression and purification

authors： Li Y,Li X,Wang G

更新日期：2006-06-01 00:00:00

abstract：:Notch signaling plays a key role in cell differentiation and is very well conserved from Drosophila to humans. Ligands of Notch receptors are type I, membrane spanning proteins composed of a large extracellular region and a 100-150 residue cytoplasmic tail. We report here, for the first time, the expression, purificat...

journal_title：Protein expression and purification

authors： Popovic M,Coglievina M,Guarnaccia C,Verdone G,Esposito G,Pintar A,Pongor S

更新日期：2006-06-01 00:00:00

abstract：:A process for bacterial expression and purification of the recombinant major wasp allergen Antigen 5 (Ves v 5) was developed to produce protein for diagnostic and therapeutic applications for type 1 allergic diseases. Special attention was focused on medium selection, fermentation conditions, and efficient refolding p...

journal_title：Protein expression and purification

authors： Kischnick S,Weber B,Verdino P,Keller W,Sanders EA,Anspach FB,Fiebig H,Cromwell O,Suck R

更新日期：2006-06-01 00:00:00

abstract：:A full-length hexokinase cDNA was cloned from Solanum chacoense, a wild relative of the cultivated potato. Analysis of the predicted primary sequence suggested that the protein product, ScHK2, may be targeted to the secretory pathway and inserted in the plant plasma membrane, facing the cytosol. ScHK2 was expressed as...

journal_title：Protein expression and purification

authors： Claeyssen E,Wally O,Matton DP,Morse D,Rivoal J

更新日期：2006-05-01 00:00:00

abstract：:Rotavirus VP8* subunit is the minor trypsin cleavage product of the spike protein VP4, which is the major determinant of the viral infectivity and neutralization. To study the structure-function relationship of this fragment and to obtain type-specific reagents, substantial amounts of this protein are needed. Thus, fu...

journal_title：Protein expression and purification

authors： Favacho AR,Kurtenbach E,Sardi SI,Gouvea VS

更新日期：2006-04-01 00:00:00

abstract：:Molecular chaperones are integral components of the cellular machinery involved in ensuring correct protein folding and the continued maintenance of protein structure. An understanding of these ubiquitous molecules is key to finding cures to protein misfolding diseases such as Alzheimer's and Creutzfeldt-Jacob disease...

journal_title：Protein expression and purification

authors： Nicoll WS,Boshoff A,Ludewig MH,Hennessy F,Jung M,Blatch GL

更新日期：2006-03-01 00:00:00

abstract：:Recombinant barley high pI alpha-glucosidase was produced by high cell-density fermentation of Pichia pastoris expressing the cloned full-length gene. The gene was amplified from a genomic clone and exons (coding regions) were assembled by overlap PCR. The resulting cDNA was expressed under control of the alcohol oxid...

journal_title：Protein expression and purification

authors： Naested H,Kramhøft B,Lok F,Bojsen K,Yu S,Svensson B

更新日期：2006-03-01 00:00:00

abstract：:In this report, we describe an optimized system for the efficient overexpression, purification, and refolding of secreted bacterial proteins. Candidate secreted proteins were produced recombinantly in Escherichia coli as Tobacco Etch Virus protease-cleavable hexahistidine-c-myc eptiope fusion proteins. Without regard ...

journal_title：Protein expression and purification

authors： Geisbrecht BV,Bouyain S,Pop M

更新日期：2006-03-01 00:00:00

abstract：:Human paraoxonase (hPON3) is a high density lipoprotein-related glycoprotein with multi-enzymatic properties and antioxidant activity which is proposed to participate in the prevention of low density lipoprotein (LDL) oxidation. In this study, hPON3 gene was amplified from Human Fetal Liver Marathon-Ready cDNA and exp...

journal_title：Protein expression and purification

authors： Lu H,Zhu J,Zang Y,Ze Y,Qin J

更新日期：2006-03-01 00:00:00

abstract：:The covalent addition of 4-amino-4-deoxy-L-arabinose (L-Ara4N) groups to lipid A, which resides in the outer membranes of bacteria such as Salmonella typhimurium and Escherichia coli, is the final step in the polymyxin-resistance pathway in these organisms. This modification is catalyzed by the inner membrane protein ...

journal_title：Protein expression and purification

authors： Bretscher LE,Morrell MT,Funk AL,Klug CS

更新日期：2006-03-01 00:00:00

abstract：:Human dermcidin, an anionic antimicrobial peptide expressed in the pons of the brain and the sweat glands, displays antimicrobial activity against pathogenic microorganisms such as Staphylococcus aureus and Candida albicans. Here, we describe the recombinant production of a 48 amino acid dermcidin variant with C-termi...

journal_title：Protein expression and purification

authors： Cipáková I,Gasperík J,Hostinová E

更新日期：2006-02-01 00:00:00

abstract：:The ubiquitin system represents a selective mechanism for intracellular proteolysis in eukaryotic cells that involves the sequential activity of three enzymes, ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2), and ubiquitin-protein ligase (E3). The identification of these proteins and their cellular...

journal_title：Protein expression and purification

authors： Reyes LF,Sommer CA,Beltramini LM,Henrique-Silva F

更新日期：2006-02-01 00:00:00

abstract：:The transcription factor IIIC (TFIIIC) is a multisubunit DNA-binding factor required for promoter recognition and TFIIIB assembly on tRNA genes transcribed by RNA polymerase III. Yeast TFIIIC consists of six subunits, organized in the two globular subcomplexes tauA and tauB, which recognize two internal tDNA promoter ...

journal_title：Protein expression and purification

authors： Mylona A,Acker J,Fernández-Tornero C,Sentenac A,Müller CW

更新日期：2006-02-01 00:00:00

abstract：:A functional IP10-scFv fusion protein retaining the antibody specificity for acidic isoferritin and chemokine function was produced at high level in Esherichia coli (E. coli). IP10-scFv gene from the recombinant plasmid pc3IP104c9 was subcloned into pET28a fused to N-terminal His-tag sequence in frame and overexpresse...

journal_title：Protein expression and purification

authors： Guo JQ,Li QM,Zhou JY,Zhang GP,Yang YY,Xing GX,Zhao D,You SY,Zhang CY

更新日期：2006-01-01 00:00:00

abstract：:Protein phosphatase 2A (PP2A) contains a 36-kDa catalytic subunit (PP2Ac), a 65-kDa structural subunit (PR65/A), and a regulatory B subunit. The core enzyme consists of the structural and catalytic subunits. The catalytic subunit exists as two closely related isoforms, alpha and beta. Several natural toxins, including...

journal_title：Protein expression and purification

authors： Ikehara T,Shinjo F,Ikehara S,Imamura S,Yasumoto T

更新日期：2006-01-01 00:00:00

abstract：:Granulocyte-macrophage colony-stimulating factor (GM-CSF) regulates proliferation, differentiation, and function of hematopoietic progenitor cells. Aside from expansion of hematopoietic cells, GM-CSF has shown efficacy in other diseases, including Crohn's disease. While GM-CSF being clinically used in humans, the abil...

journal_title：Protein expression and purification

authors： Sainathan SK,Tu L,Bishnupuri KS,Han M,Li A,Newberry RD,McDonald KG,Crimmins DL,Houchen C,Anant S,Dieckgraefe BK

更新日期：2005-12-01 00:00:00

abstract：:Accurate diagnosis is essential for the treatment, prevention, and control of tuberculosis. Poor specificity of the tuberculin skin test in BCG-vaccinated populations and constraints to implementation of PCR and CMI-based diagnostic assays in developing countries warrant development of easy-to perform robust serologic...

journal_title：Protein expression and purification

authors： Kulshrestha A,Gupta A,Verma N,Sharma SK,Tyagi AK,Chaudhary VK

更新日期：2005-11-01 00:00:00

abstract：:An active form of a single-chain antibody fragment (scFv) from the murine monoclonal antibody ABL-1, which is specific for B-cell-activating factor of the TNF family, was produced in Escherichia coli. The complementary DNAs encoding the variable regions of the heavy chain (VH) and light chain (VL) were connected by a ...

journal_title：Protein expression and purification

authors： Cao P,Tang XM,Guan ZB,Diao ZY,Zhang SQ

更新日期：2005-10-01 00:00:00

abstract：:The B-subunits of replicative DNA polymerases belong to the superfamily of calcineurin-like phosphoesterases and are conserved from Archaea to humans. Recently we and others have shown that the B-subunit (DP1) of the archaeal family D DNA polymerase is responsible for proofreading 3'-5' exonuclease activity. The simil...

journal_title：Protein expression and purification

authors： Jokela M,Raki M,Heikkinen K,Sepponen K,Eskelinen A,Syväoja JE

更新日期：2005-09-01 00:00:00

abstract：:A fibrinolytic enzyme was purified from the cultured mycelia of Armillaria mellea by ion-exchange chromatography followed by gel filtration, and was designated A. mellea metalloprotease (AMMP). The purification protocol resulted in a 627-fold purification of the enzyme, with a final yield of 6.05%. The apparent molecu...

journal_title：Protein expression and purification

authors： Lee SY,Kim JS,Kim JE,Sapkota K,Shen MH,Kim S,Chun HS,Yoo JC,Choi HS,Kim MK,Kim SJ

更新日期：2005-09-01 00:00:00

abstract：:Flt3 ligand (FL) is a potent hematopoietic cytokine that affects the growth and differentiation of hematopoietic progenitor and stem cells both in vivo and in vitro. Pichia pastoris transformants secreting high-level rhFL were obtained using 'yeastern blotting' method and the expression level in liquid was about 30 mg...

journal_title：Protein expression and purification

authors： Zhang YL,Chen SS,Yang KG,Su L,Deng YC,Liu CZ

更新日期：2005-08-01 00:00:00

abstract：:This is the first report of an insect esterase efficiently expressed in the methylotrophic yeast Pichia pastoris (so far insect esterases have been produced only in the baculovirus system). Having isolated a Tribolium castaneum carboxylesterase cDNA (TCE), we were initially unable to express it in Escherichia coli or ...

journal_title：Protein expression and purification

authors： Delroisse JM,Dannau M,Gilsoul JJ,El Mejdoub T,Destain J,Portetelle D,Thonart P,Haubruge E,Vandenbol M

更新日期：2005-08-01 00:00:00

abstract：:Previously, the lysozyme gene of the Klebsiella phage K11 was partially sequenced in our lab. Using the sequence information the lysozyme gene of the Klebsiella phage K11 was amplified and cloned using the polymerase chain reaction of the pfu DNA polymerase. The nucleotide sequence of phage K11 lysozyme gene was deter...

journal_title：Protein expression and purification

authors： Junn HJ,Youn J,Suh KH,Lee SS

更新日期：2005-07-01 00:00:00

abstract：:Recombinant baculoviruses have proved to be a very useful means to express many proteins over the last 20 years. Since their introduction, there have been a number of significant improvements that have simplified and speeded up the construction of baculoviruses. One of the most commonly used methods relies upon recomb...

journal_title：Protein expression and purification

authors： McCall EJ,Danielsson A,Hardern IM,Dartsch C,Hicks R,Wahlberg JM,Abbott WM

更新日期：2005-07-01 00:00:00

abstract：:HSPC144 is a newly identified gene in human CD34(+) hematopoietic stem/progenitor cells. In this work, we have expressed and purified the 225-residue protein from Escherichia coli BL21 (DE3) and identified a stable fragment HSPC144-P (residues 44-225) by limited proteolysis method. The HSPC144-P fragment exhibits high...

journal_title：Protein expression and purification

authors： Song AX,Chang YG,Gao YG,Lin XJ,Shi YH,Lin DH,Hang QH,Hu HY

更新日期：2005-07-01 00:00:00

abstract：:Endostatin, a 20kDa C-terminal fragment of collagen XVIII, is a potent anti-angiogenic protein and inhibitor of tumor growth. Recombinant endostatin was prepared from Escherichia coli deposited as insoluble, inactive inclusion bodies. In the present study, we produced soluble and biologically active recombinant human ...

journal_title：Protein expression and purification

authors： Xu HM,Zhang GY,Ji XD,Cao L,Shu L,Hua ZC

更新日期：2005-06-01 00:00:00

abstract：:The murine monoclonal antibody 125E11 is an IgG which recognizes PreS1(21-47) fragment of large hepatitis B surface antigen. It has been successfully used for clinical detection of HBV virion in serum of hepatitis B patients. In present study, the genes of variable region in heavy chain (VH) and light chain (VL) of 12...

journal_title：Protein expression and purification

authors： Yang X,Hu W,Li F,Xia H,Zhang Z

更新日期：2005-06-01 00:00:00

abstract：:Post-translational modification of proteins is a dynamic way of generating new protein-protein interaction interfaces that are critical for signaling networks in diverse cellular functions. Purified recombinant proteins frequently lack these signature modifications. Using the tumor suppressor p53 as the model protein,...

journal_title：Protein expression and purification

authors： Acharya A,Xu XJ,Husain-Ponnampalam RD,Hoffmann-Benning S,Kuo MH

更新日期：2005-06-01 00:00:00

abstract：:The expression of recombinant proteins in bacterial hosts may alter the biophysical properties of the protein of interest as a result of differences in post-translational processing from that observed when produced in the native cell. For example, recombinant human interleukin-1beta (IL-1beta) is produced as three iso...

journal_title：Protein expression and purification

authors： Covalt JC Jr,Cao TB,Magdaroag JR,Gross LA,Jennings PA

更新日期：2005-05-01 00:00:00

abstract：:To obtain strong bacterial expression of proteins that seem to be hard to express in bacteria or are highly toxic for bacteria, it is possible to create a palette of similar constructs, differing only by several nucleotides, gradually deleted from the full-length clone by exonuclease III. When a construct is equipped ...

journal_title：Protein expression and purification

authors： Pecenková T,Filigarová M,Cerovská N

更新日期：2005-05-01 00:00:00

abstract：:Glutathione S-transferase (GST) fusion proteins are widely used in protein production for pure immunogens, protein-protein, and DNA-protein interaction studies. Using basic pGEX vectors, foreign DNA is introduced to the C-terminus of the GST gene and the produced fusion proteins are C-terminally orientated. However, b...

journal_title：Protein expression and purification

authors： Aatsinki JT,Rajaniemi HJ

更新日期：2005-04-01 00:00:00

abstract：:The full-length cDNA that encodes the hemolytic toxin Avt-I, with 226 amino acids, from the venomous sea anemone Actineria villosa has been cloned using the oligo-capping method. The cDNA contains 681bp open reading frame and its predicted amino acid sequences revealed that Avt-I was basic polypeptides without cystein...

journal_title：Protein expression and purification

authors： Uechi G,Toma H,Arakawa T,Sato Y

更新日期：2005-04-01 00:00:00

abstract：:A genomic DNA of 1416 bp containing an open reading frame encoding a manganese superoxide dismutase (Mn-SOD) from Tatumella ptyseos ct was cloned. Sequence analysis of this new gene revealed that it translates 205 amino acid residues. The deduced amino acid sequence showed variable identities (41-91%) with sequences o...

journal_title：Protein expression and purification

authors： Ken CF,Lee CC,Duan KJ,Lin CT

更新日期：2005-03-01 00:00:00

abstract：:The major immunodominant integral outer membrane protein C (OmpC) from Salmonella typhi Ty21a was overexpressed, without the signal peptide, in Escherichia coli. The protein aggregates as inclusion bodies (IBs) in the cytoplasm. OmpC from IBs was solubilized with 4 M urea and refolded. This involved rapid dilution of ...

journal_title：Protein expression and purification

authors： Kumar PD,Krishnaswamy S

更新日期：2005-03-01 00:00:00

abstract：:Enzymatic hydrolysis of the N-iminylamide was investigated in this study. An enzyme possessing N-iminylamidase activity from pig liver was purified to electrophoretic homogeneity. This enzyme was also active, however, with imides and appears to be identical to pig liver imidase. The identification was confirmed by cop...

journal_title：Protein expression and purification

authors： Huang CY,Yang YS

更新日期：2005-03-01 00:00:00

abstract：:We have previously reported that a variety of solid human tumor cell lines express a large number of receptors for interleukin-13 (IL-13). These receptors could be targeted with a chimeric fusion protein consisting of human IL-13 and a truncated form of Pseudomonas exotoxin (PE). We describe here optimization of criti...

journal_title：Protein expression and purification

authors： Joshi BH,Puri RK

更新日期：2005-02-01 00:00:00